At just before 8:30 p.m., emergency crews responded to the intersection of Pulaski Highway and Allender Road for a report of a serious accident.
At the scene, paramedics found that a female pedestrian had been struck.
The victim was pronounced dead at the scene. The driver remained at the scene and showed no visible signs of impairment, according to police.
Route 40 is now shut down for crash investigation purposes.
Motorists should avoid the area.